Fig. 6. Tßh gene localization in honey bee brains revealed by in situ hybridization. In situ hybridization was performed on nurses and foragers using 10 µm sections but only brains from nurses are shown here. Control experiments using DIG-labeled sense probes gave no significant signals in any of the in situ hybridization experiments (data not shown). (A) Frontal view of anterior (left), middle (middle) and posterior portion of the section (right). Areas corresponding to B-G are boxed. (B-G) Signals are indicated by arrows. m, medulla; lo, lobula; AL, antennal lobe; OES, esophagus; SOG, subesophageal ganglion. ICa, mushroom body lateral calyces; MCa, mushroom body medial calyces. Bars indicate 100 µm.