Fig. 4. Nucleotide and deduced amino acid sequence of Apis T ßh. The first nucleotide and amino acid residue of the translational start site are designated as position 1. The amino acid positions of DOMON and Cu²⁺ type II ascorbate-dependent monoxygenase domains predicted by Scansite 2.0 (Obenauer et al., 2003) are indicated by italic bold and bold text, respectively. The nucleic acid sequences in white text on a black background are the regions used to synthesize the DIG-labeled antisense RNA probe for aTßh in situ hybridization procedures. The nucleic acid sequences used as primers for quantitative real-time PCR analysis are shown in black text on a grey background and the sequence used as a probe is shown in underlined black text on a grey background.