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Figure 8


Fig. 8. Protein disulfide isomerase (PDI) mRNA (filled symbols) and protein (open symbols) in the (A) gill and (B) white muscle from Gillichthys mirabilis. Average protein concentrations and mRNA levels are shown for fish (N=4) from each time point, either during heat shock (solid lines; circles) or during recovery (broken lines; squares). Methods were identical to those described in the legend to Fig. 5, except that the antibodies used were specific to PDI. Average pixel intensity for each spot was normalized to the average for the four time 0 individuals. For protein levels, values are means ± s.d (N=4). *Heat shock time points that differ significantly (ANOVA, (P ≤0.001) from time 0; {ddagger}recovery time points that differ significantly from time 0. In some cases, error bars are contained within symbols.