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Figure 6


Fig. 6. Hsp70 mRNA (closed symbols) and protein (open symbols) in (A) the gill and (B) white muscle from Gillichthys mirabilis. Average protein concentrations and mRNA levels are shown for fish (N=4) from each time point, either during heat shock (solid lines; circles) or during recovery (dashed lines; squares). Methods were identical to those described in the legend to Fig. 5, except that antibodies used were specific to Hsp70. Average pixel intensity for each spot was normalized to the average for the four time 0 individuals. For protein levels, values are means ± s.d. (N=4). *Heat shock time points that differ significantly (ANOVA, P ≤0.001) from time 0; {ddagger}recovery time points that differ significantly from time 0. In some cases, error bars are contained within symbols.