Fig. 4. Ubiquitin-conjugated proteins in gill and white muscle from Gillichthys mirabilis. For determination of the concentration of ubiquitin-conjugated proteins, thawed tissues were homogenized and dot-blotted onto nitrocellulose membranes. Following incubation in primary and secondary antibodies, blots were visualized with enhanced chemiluminescence reagent and exposed to X-ray film; exposed film was scanned and spot intensity quantified densitometrically. Average pixel intensity for each spot was normalized to the average for the four time 0 individuals. Values are means ± s.d. (N=4). ^*Heat shock time points, in gill tissue, that differ significantly (ANOVA, P 0.001) from time 0; recovery time points, in gill tissue, that differ significantly from time 0; heat shock time points, in muscle tissue, that differ significantly (P 0.001) from time 0. In some cases, error bars are contained within symbols.