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Figure 6


Fig. 6. Chromatography of CuquEndo hydrolysis of different nucleic acid substrates on an IECDEAENPR column. (A) PolyT standard used to calibrate the column. Peaks represent polymers of 20, 15, 12, 10, 9, 8, 7, 6, 5, 4, 3 and 2 nucleotides. (B,C) The effect of incubation of double-stranded plasmid DNA with (B) salivary gland extract or (C) recombinant enzyme-containing supernatant. Both enzyme sources hydrolyzed the plasmid substrate with no sequence specificity. (D,E) Negative controls are (D) undigested double-stranded plasmid DNA and (E) recombinant enzyme-containing supernatant alone.