Fig. 3. (A,B) In vitro effects of different concentrations of H89 on the percentage germinal vesicle breakdown (GVBD) in Heteropneustes fossilis. (A) H89 alone or co-incubated with 2-hydroxyestradiol-17ß (2-OHE₂, 5 µmol l^–1) for 24 h. Values are mean ± s.e.m. (N=3). (B) The effect of H89 on IBMX inhibition of 2-OHE₂-induced GVBD. Data were analyzed by one-way ANOVA (P<0.001) and Newman–Keuls' test (P<0.05). Asterisks indicate significant difference from the vehicle control (VC) group. Letters indicate comparisons with the 2-OHE₂ (A) or H89 (B) group. Numbers show comparisons with 2-OHE₂ (B) group. Groups marked with the same letter or number are not significantly different and that with different letters or numbers are significantly different. NS, not significant from the vehicle control (VC) group.