Fig. 7. Nrf2 nuclear translocation is related to the administered dose of paracetamol. Mice were treated with the indicated doses of paracetamol. After 1 h they were sacrificed, livers were removed, nuclei prepared and nuclear proteins extracted. These were separated using SDS–PAGE, alongside a recombinant mouse Nrf2 positive control, transferred to a nitrocellulose membrane, probed for Nrf2 using a polyclonal anti-Nrf2 antiserum and visualised using chemiluminescence. Each data point in the blot and in the response-curve is obtained from pooled extracts of five animals (+ s.d.).