Fig. 1. Hippocampal slices in an interface chamber. (A) Diagram of a mouse brain showing the positions of the hippocampus and a transverse hippocampal slice. (B) Circuitry and anatomical subregions within a hippocampal slice. (C) Schematic diagram of an interface recording chamber. Hippocampal slices are placed on meshed rings positioned within individual wells. This temperature-regulated chamber allows slices to be perfused with artificial cerebrospinal fluid (ACSF) while exposed to a humidified, oxygenated environment. Adapted from (Young, 2005).