Fig. 6. Myogenin (myog) expression in embryos of the tiger pufferfish (Takifugu rubripes) reared at 15°C (open circles), 18°C (closed circles) and 21°C (closed triangles). The eggs were from a single cross. mRNA transcripts were measured by qPCR using 18S rRNA as an endogenous control. The results were normalised against the highest expression value (21°C, 40 h.p.f.) and plotted against somite interval (development time/divided by the time to form one somite pair) in an attempt to normalise developmental stage at the different temperatures. The results represent mean ± s.e.m. of 4 batches of embryos per temperature. From (Fernandes et al., 2006).