Fig. 3. Effect of agonist-induced depletion of cytosolic Ca²⁺ stores on tBHP-evoked Ca²⁺ increase in pancreatic acinar cells. Cells were perfused with either 1 nmol l^-1 CCK-8 (A) or 1 µmol l^-1 thapsigargin (TPS) (B) in Ca²⁺-free medium, followed by 1 mmol l^-1 tBHP. Traces are representative of 74 and 58 such cells taken from 12 and 10 different experiments, respectively. (C) Histogram of the mean post-stimulus [Ca²⁺]_c under different experimental conditions in 5-12 independent experiments. Values are means ± s.e.m. ^*P<0.05.