Fig. 1. tBHP-evoked [Ca²⁺]_c increase in isolated rat pancreatic acinar cells. Cells were perfused with 1 mmol l^-1 tBHP in (A) normal-Ca²⁺ or (B) Ca²⁺-free (containing 1 mmol l^-1 EGTA) medium. (C) Cells were perfused with 1 mmol l^-1 tBHP followed by 2 mmol l^-1 DTT in Ca²⁺-free medium. Traces are representative of 61 and 53 such cells taken from 10 and 8 different experiments, respectively. (D) Histogram represents the mean post-stimulus [Ca²⁺]_c under different experimental conditions of 8-10 independent experiments. Values are means ± s.e.m. ^*P<0.05.