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Figure 4


Fig. 4. Immunolocalisation of Mvl in both the larval and adult alimentary canal. (A–C) Larval anterior midgut. (A) Mvl staining within the larval anterior midgut. (B) Higher magnification reveals punctuate subcellular staining within enterocytes (arrow). (D–F) Mvl-positive staining within the adult posterior midgut. (D) Staining within these enterocytes appears to be diffuse (arrow), with some punctate staining. (E) Small scattered cells show strong Mvl staining (arrow). (G–I) Mvl localisation within the Malpighian tubules. (G) Diffuse staining within principal (type 1) cells (arrow). (H) Strong Mvl-positive staining within small neuroendocrine-like cells (arrow). (C,F,I) Mvl peptides confirm staining specificity in each cell type. Scale bars, 200 µm (A,C); 100 µm (B,D–I).