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Figure 6


Fig. 6. Effect of 10–4 mol l–1 DIDS on fluid secretion rate, transepithelial potential (TEP), and intracellular pH in 10–6 mol l–1 5-HT stimulated Malpighian tubules. (A) Fluid secretion rate (mean ± s.e.m., N=9). Tubules were exposed to DIDS (filled symbols) at the point indicated by the arrow, or to the vehicle alone (open symbols).(B) Representative TEP recording. The first arrow indicates the addition of 5-HT 10–5 mol l–1. The second arrow indicates the time of exposure to DIDS. (C) Intracellular pH before and 2 min after addition of 10–4 mol l–1 DIDS (mean + s.e.m., N=6).