Fig. 5. (A) Representative and (B) mean tissue distributions of epithelial calcium channel (ECaC) in rainbow trout (Oncorhynchus mykiss) as determined through RT–PCR and real-time RT–PCR, respectively. ECaC-QPCR1 and ECaC-QPCR2 primers were used for PCR detection of ECaC, and ß-actin-FWD and ß-actin-REV primers were used for the detection of ß-actin (see Table 1 for primer sequences). For each tissue, a no template control (NTC) sample was tested (data not shown for real-time PCR results). For real-time PCR, the results are presented as the expression of ECaC relative to ß-actin and standardised to ECaC expression in the gill (N=4).