Fig. 8. A peptide directed against the interaction domain between TRPC2 and IP₃R3 blocks the chemosignal-activated currents in S. odoratus vomeronasal sensory neurons (VSNs) when included in the patch pipette in the whole-cell configuration. (A) Amino acid sequence of the synthesized peptide (derived from mTRPC2, amino acids 905–934) (Tang et al., 2001). (B) Histogram plot of the chemosignal-activated current over 10 min when 10 µmol l^–1 peptide is included in the recording pipette. The normal chemosignal-activated current over time is denoted by a broken line (see Fig. 4). Current obtained for each cell at varying time points (t_n) was normalized to its first exposure to chemosignal (t₀) Asterisks denote significant differences between control and peptide, two-way repeated measures ANOVA, followed by SNK pairwise multiple comparison between treatment and time, P0.05). (C) Representative example of peptide treatment. While in the whole-cell configuration, the VSN was presented with a chemosignal, and a baseline (immediately after the whole-cell configuration was obtained) recording was made (top). The same chemosignal-activated current is shown 10 min after peptide infusion (bottom). Broken line denotes baseline current.