Fig. 2. Quantification of mRNA levels in hatchling larvae by qRTPCR. (A–C) Mean gene Ct values for (A) Rev8/+ vs Rev8/Rev8 (N=5), (B) dimm^KG02598/Rev4 vs dimm^KG02598/+ (N=6) and (C) crc¹/crc¹ vs crc¹/+ larvae (N=5). The N values represent the number of independent mRNA extractions. (A'–C') Levels of transcripts in homozygous or transheterozygous mutants in A–C expressed as a percentage of the levels in heterozygous controls. During each cycle of the qRTPCR, the Ct value increases by 1 as the quantity of qRTPCR product is doubled. Therefore, the percentage change in each mRNA shown in A'–C' was calculated as 1/2^{(Ct experimental–Ct control)}. *P<0.05; **P<0.01; ***P<0.001; one-way ANOVA, sequential Bonferroni post-hoc test.