Fig. 5. Changes in the accumulated mRNA levels of carp MYH isoforms during ontogeny. Total RNAs (20 µg) were electrophoresed in a 1% agarose gel and transferred on to the nylon membranes, which were then hybridized with ³²P-labeled probe. Lanes contain total RNAs from the whole embryos at 30 h, 42 h, 61 h, and 77 h.p.f., from the whole larvae at hatching (96 h), from the muscle of juveniles aged 1 and 7 months post-hatching and from the fast and slow skeletal muscles of thermally acclimated adult fish. An ethidium bromide-stained gel shows 28S and 18S rRNA. Consensus indicates the membrane hybridized with the probe, which is supposed to react with all skeletal MYH mRNA of carp. Each lane contains 5 µg of total RNA.