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Fig. 5. Interaction of apoA-I with brush border membrane vesicles (BBMV). (A) 125I-apoA-I bound to BBMV; S, washed peripheral membranes; P, integral membrane fractions. Fractions were separated on a 12% SDS polyacrylamide gel and the protein detected by autoradiography. (B) Endogenous content of apoA-I in BBMV and distribution in the peripheral membrane (S) and integral membrane (P) fractions analysed by western blot.