Fig. 4. Concentration and temperature effect on apoA-I binding to brush border membrane vesicles (BBMV). (A) BBMV were incubated with varying concentrations of ¹²⁵I-apoA-I. The amount of bound apoA-I was determined as described in Materials and methods. Total apoA-I binding (circles) and non-specific binding (triangles) was determined by adding 100 times the molar ratio of unlabelled apoA-I to the binding assay. Values are means ± S.E.M. of triplicate determinations. (B) BBMV were incubated with 200 nmol l^{–1 125}I-apoA-I at different temperatures. Values are means ± S.E.M. of triplicate determinations.