Fig. 3. apoA-I gene expression in carp intestine. (A) Total RNA (30 µg) extracted from liver and each of the intestinal segments were analysed by northern blot. Lane 1, liver RNA; lanes 2–8, all segments of intestine, from proximal to distal. (B) Ethidium bromide-stained total RNA (30 µg) from liver (lane 1) and all intestinal segments (lanes 2–8). (C) RT–PCR products for apoA-I (top) and ß-actin (bottom) gene expression. Lanes 1, 2, liver RNA with and without reverse transcriptase, respectively; lanes 3–9, intestinal RNA from all segments.