Fig. 5. Water loss (at 25°C) from batches of 2 day old Manduca sexta eggs. Each batch contained 40 eggs. The first few minutes of each trace represent a baseline reading (air stream directed through an empty chamber). Air streams were then redirected through the egg-containing chamber (black arrow) followed by another baseline reading (colored arrows). During the second baseline, batches were extracted for 1 min in 100% ethanol or 2:1 (v:v) chloform:methanol, blotted dry and returned to their chambers. A control batch was not extracted. The air stream was then directed again through the egg-containing chamber (black asterisk), after which a final baseline reading (colored asterisks) was taken.