Fig. 3. Desensitization of Drosophila DH31-R1 requires accessory proteins. (A) Two typical HEK cells following transfection with ß-Arr2-GFP, RAMP1, RCP and CG17415, prior to peptide application. The cells display a uniform cytoplasmic distribution of fluorescence. (B) A different cell 20 min following application of 1 µmol l^–1 DH₃₁. Note prominent rearrangement of fluorescence in large vesicles throughout the cell. Cells were transfected with DNA at a ratio of 5 (CG17415): 1 (RAMP1): 1 (RCP): 1 (ßarr2-GFP).