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Fig. 10. Immunoblot of the stress-associated proteins OSR1 (A) and SPAK (B), comparing expression in freshwater (FW)- and seawater (SW)-acclimated animals. Quantitative western analysis revealed significantly higher level of OSR1 and SPAK expression in FW- as compared to SW-acclimated animals for the gill and opercular epithelium (oe; N=3, *P<0.05) with the difference in OSR1 expression being more marked than that for SPAK.