Fig. 2. (A) The expression of Na+,K+-ATPase (NKA) mRNA in the
gill was determined using the antisense probe and was found in distinct cells
on the primary filament and lamellae (large arrowheads). (B) The sense
Na+,K+-ATPase mRNA probe revealed no staining. (C,E) The
Na+,K+-ATPase mRNA and protein were colocalized within
the same cells (small arrows). (D,F) Double labeling for
Na+,K+-ATPase mRNA and nNOS revealed colocalization of
these enzymes (arrowheads). Scale bar, 100 µm.