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Fig. 1. (A-D) The presence of NO-producing cells (A-C) was compared with that of Na+,K+-ATPase (NKA) {alpha}-subunit distribution (D) in the gill of Atlantic salmon. nNOS (A) and eNOS (B) immunoreactive cells was mainly restricted to large cells at the base of and along the secondary lamellae (large arrowheads) and in the interlamellar spaces of the primary filaments. Only small cells located deep in the filament were iNOS immunoreactive (C, small arrowheads). (E) NADPHd histochemistry revealed strong staining of large cells similar to the staining with nNOS and eNOS. In addition, a distinct NADPHd punctuate staining (arrows) and band staining (*) were observed on the lamellae (E). Scale bar, 100 µm.