Fig. 6. Body composition of larvae of P. marioni exposed five times to –5.5°C, and that froze between 0 and 5 times during those exposures. Values are least-square means ± 95% confidence intervals of mass (g) measurements. Gut water differed significantly with frequency of freezing; significant differences are indicated by different letters above the bars. See Table S2 in supplementary material for statistics and covariates used in ANCOVA.