Fig. 2. Body composition in Pringleophaga marioni larvae exposed to increasing numbers of freeze–thaw cycles (filled squares, dotted lines) or handling controls (open triangles, solid lines), and 5 (r-5) and 30 (r-30) days after the final exposure. Values are least-square means ± 95% confidence intervals of nine measurements. T indicates a significant difference between treatment and control, TxE indicates a significant treatment x exposure interaction. See Table S1 in supplementary material for statistics and covariates used for each analysis.