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Fig. 2. Representative in-series fascicle segment strain recordings obtained at the anterior and middle SB pectoralis sites for two wingbeats during slow (~4-5 m s-1) level flight, together with the EMG recorded from the distal location in each instance. EMG signals were bandpass filtered from 40 to 1500 Hz and sonomicrometer fascicle strain recordings were low-pass (40 Hz) filtered using a 4th order zero-lag digital Butterworth filter. In general, the pattern of fascicle strain (P>0.05 for time of maximum and minimum strain) and timing of EMG activation (P>0.05) were consistent across proximal and distal sites and at both locations among the birds sampled. In-series fascicle strain magnitude was also generally similar (in the cases shown for pigeon 3, distal fascicle strain averaged 3.5% less than that recorded from the proximal site), with differences in strain magnitude averaging less than 3.8% when proximal and distal sites were compared across all individuals at both locations sampled (see Table 2).