Fig. 6. (A) Effect of 10 µmol l^-1 bumetanide (added in the mucosal bath) on the V_te response to hypotonic stress expressed as V_te ( values were calculated as the differences between the V_te and I_sc values after hypotonic stress application and the value before; values were calculated every 5 min during the time course of the exposure). Time=0 indicated the start of the exposure to hypotonic stress. Data are reported as mean ± S.E.M. of 5 separate experiments. (B) Effect of 1 mmol l^-1 ouabain (added in the serosal bath) on the V_te response to hypotonic stress. Values are means ± S.E.M. of 4 experiments. The statistical significance of the differences was tested by paired Student t-test. ^*P<0.05.