Fig. 6. Hyperacetylation of histone H3 following HDAC inhibitor treatment and heat shock induction. (A,B) Western blots of the acetylated histone H3 (AcH3) in long-lived and in short-lived flies, respectively. The results of photodensitometric analysis of A and B are shown in C. The upper bands are the internal reference actin, and the lower bands are acetylated histone H3. Values shown are the mean ± S.D. of three independent experiments. ^*Significant (P<0.05); ^**highly significant (P<0.01). Abbreviations as in Fig. 5.