Fig. 2. Difference in basal expression of hsp22 (A) and hsp70 (B) genes during aging between long- and short-lived lines. Total RNA was isolated from flies, which were cultured and treated as described in the Materials and methods until 6 days and 30 days. Quantitative real-time PCR was performed to determine the mRNA expression levels of hsp genes (N=3). Values are means ± S.E.M.