Fig. 1. Diagram of the epi-microspectrophotometric apparatus. The monochromatic
beam contains: Qm, Ealing 150 W optically stabilized Xenon-arc
illuminator; Fs, heat filter and stray light filter; G,
computer-controlled monochromator with entrance slit An and exit
slit Ax; Lm, quartz condenser; Af, field
aperture; W, computer-controlled counter-rotating neutral density wheels;
Sm, computer-controlled Uniblitz shutter; M, mirror. The adapting
beam contains: Qa, 45 Wquartz-iodine illuminator; Fh,
heat filter; Fd, neutral filter; Fc, cut-off filter;
La, telephoto lens; Aa, field aperture; Sa,
Uniblitz shutter. The two collimated beams are combined in quartz
beam-splitter Bq and inserted into the Leitz Pol-Opak
epi-illuminator equipped with 620-257 illumination slide designed for low
stray-light. A, aperture diaphragm; Lo, microscope objective,
either Leitz 8X/0.18P acromat/215 or Zeiss Luminar-25 mm/0.15; Le,
GF10X eye piece; E, eye of completely intact butterfly mounted on Leitz UT-4
goniometric stage. The Leitz UV-MPV photometer head contains Ap,
adjustable/centrable photometer diaphragm; Fp, stimulus-blocking
filter; P, cooled Hammamatsu R928 photomultiplier.