Fig. 4. Western blot analysis of 2DE reveal normal temporal phosphorylation of flightin in transgenic line. (A,C,E) wild type; (B,D,F) P{fln⁺}0.2. A and B, P15 pupa; C and D, <1 h post-eclosion; E and F, 2–4-day-old adult. For all blots, the basic end is to the left. 2D gel analysis was followed by immunoblot analysis using anti-flightin polyclonal antibody. N1 and N2 are the non-phosphorylated isoelectic variants while P1 through P9 are phosphorylated variants (see Vigoreaux and Perry, 1994). For each of the developmental stages, P{fln⁺}0.2 transgenic flies have a nearly identical phosphorylation pattern when compared to the wild type.