Fig. 3. Western blot of flightin levels throughout pupal stages of development. (A) P{fln⁺}0.2 (B) wild type. Pupa were staged by visual inspection using standard criteria (Bainbridge and Bownes, 1981). Accumulation of flightin in wild type begins at stage P8 (Vigoreaux et al., 1993), approx 2–8 h after accumulation in P{fln⁺}0.2-rescued flies.