Fig. 8. Four representative time courses of change in the rate of fluid discharge from the contractile vacuole after changing the external osmolarity in P. multimicronucleatum. (A) A 164 mosmol l^-1-adapted cell was subjected to 104 mosmol l^-1. (B) A 144 mosmol l^-1-adapted cell was subjected to 84 mosmol l^-1. (C) A 104 mosmol l^-1-adapted cell was subjected to 164 mosmol l^-1. (D) A 84 mosmol l^-1-adapted cell was subjected to 144 mosmol l^-1.