Fig. 6. Effects of the degree of increase in the external osmolarity on the time course of change in cell volume after increasing the external osmolarity in P. multimicronucleatum. (A) Two groups of cells adapted to 4 mosmol l^-1 were subjected to 64 mosmol l^-1 (the degree of increase; 60 mosmol l^-1, gray open circles) or to 44 mosmol l^-1 (the degree of increase; 40 mosmol l^-1; black open circles). (B) Three groups of cells adapted to 64 mosmol l^-1 were subjected to 124 mosmol l^-1 (the degree of increase; 60 mosmol l^-1, gray open circles), 104 mosmol l^-1 (the degree of increase; 40 mosmol l^-1, red open circles) or 84 mosmol l^-1 (the degree of increase; 20 mosmol l^-1, black open circles). Each plot for cell volume is the mean±S.D. (N=3) and presented as a value relative to the cell volume before increasing the external osmolarity.