Fig. 5. Effects of the degree of decrease in the external osmolarity on the time course of change in cell volume after decreasing the external osmolarity in P. multimicronucleatum. (A) Two groups of cells adapted to 144 mosmol l^-1 were subjected to 84 mosmol l^-1 (the degree of decrease; 60 mosmol l^-1, gray open circles) or to 104 mosmol l^-1 (the degree of decrease; 40 mosmol l^-1, black open circles). (B) Three groups of cells adapted to 84 mosmol l^-1 were subjected to 24 mosmol l^-1 (the degree of decrease; 60 mosmol l^-1, gray open circles), 44 mosmol l^-1 (the degree of decrease; 40 mosmol l^-1, black open circles) or 64 mosmol l^-1 (the degree of decrease; 20 mosmol l^-1, red open circles). Each plot for cell volume is the mean±S.D. (N=3) and presented as a value relative to the cell volume before decreasing the external osmolarity.