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Fig. 3. Time courses of changes in cell volume after increasing the external osmolarity by 60 mosmol l-1 in five different groups of P. multimicronucleatum adapted to different osmolarities. (A) 104 mosmol l-1-adapted cells were subjected to 164 mosmol l-1. (B) 84 mosmol l-1-adapted cells were subjected to 144 mosmol l-1. (C) 64 mosmol l-1-adapted cells were subjected to 124 mosmol l-1. (D) 24 mosmol l-1-adapted cells were subjected to 84 mosmol l-1. (E) 4 mosmol l-1-adapted cells were subjected to 64 mosmol l-1. Each plot for cell volume is the mean±S.D. (N=3) and presented as a value relative to the cell volume before increasing the external osmolarity (at time 0).