Fig. 2. Western blot analyses of human colonic mucus. For each analysis identical amounts of CA activity were applied for mucus samples and positive controls, respectively: 0.012 U ml^-1 in the case of CAI, CAII and CAVI and 0.128 U ml^-1 in the case of CAIV. Dilution of antibodies and detection were carried out as described in Materials and methods.