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Fig. 5. Effects of 8-cpt cAMP (added to both sides) on Isc. (A) Tracing showing the sustained increase in Isc observed after stimulation with 10 µmol l-1 8-cpt cAMP. (B) Effects of 10 µmol l-1 8-cpt cAMP on Isc under Cl- free conditions. Note the inhibitory effects of 10 µmol l-1 acetazolamide (an inhibitor of carbonic anhydrase activity) on the residual current. (C) Effects of Cl- (N=5) and HCO3- (N=5) substitution on the 8-cpt cAMP stimulated Isc response.