Fig. 12. Congo Red (CR) staining of hagfish slime. Slime threads (but not mucins) bound CR, but only threads from slime that was physically perturbed showed metachromasia. (A) Bright field image of slime threads from unperturbed slime. (B) Dark-field (polarisers crossed) image of same threads. Note the lack of CR metachromasia in most of the threads. (C) Bright field image of threads from perturbed slime. (D) Same as C, dark field. (E) Bright field image showing bundling of slime threads in perturbed slime. (F) Same as E, dark field. Bar, 10 µm.