Fig. 7. Detection of phosphorylation in (A) demembranated and (B) glycerol-treated
sperm treated with various solutions. 32P-labelled proteins were
separated by a 12.5% separating gel. Demembranated sperm were reactivated in
the presence of cAMP. Glycerol-treated sperm showed motility in the 100 mmol
l1 NaCl and KCl solutions. Bars on the left side show
molecular mass standards.