Fig. 5. Effect of inhibitors on glycerol-treated sperm. Sperm were incubated for 30
min in ASP containing (A) CCCP, an uncoupler of the mitochondrial electron
transport chain for ATP synthesis; (B) SQ22536, an adenylyl cyclase inhibitor;
(C) H-89, a PKA inhibitor. Gray bars, percentage motility of intact sperm;
black bars, glycerol-treated sperm. Motility was determined from video
recordings taken 5 s after motility initiation. Values are means ±
S.D. (N=3).