Fig. 6. Induction of Hsp70 expression by thermal stress and/or 1 µmol l^–1 CuCl₂ in M. galloprovincialis gill tissue. (A) Hsp70 was detected in extracts (100 µg of protein) from gill tissue of animals incubated at 30°C for 30 min either in the absence or presence of 1 µmol l^–1 CuCl₂ or at 15°C with 1 µmol l^–1 CuCl₂ in the absence or presence of 1 µmol l^–1 SB203580 for 30 min. (B) Densitometric analysis of Hsp70 bands by laser scanning. Results are means ± S.E.M. for four independent experiments performed with similar results. The molecular mass markers (kDa) are shown to the right of the panel. P<0.001, **P<0.01 versus control value.