Fig. 5. Incorporation of ³²P during motility activation of demembranated flagella (axonemes). Sperm were suspended in CC solution (CC treatment) or CC solution containing 20 mmol l^–1 NH₄Cl (NH₄Cl treatment) before the preparation of axonemes. The axonemes were diluted with the reactivation solutions (pH range 7.0–8.0) containing [-³²P]ATP, and axonemal proteins labeled with ³²P were detected. (A) ³²P-labeled proteins in the CC-treated sperm axonemes. (B) ³²P-labeled proteins in the NH₄Cl-treated sperm axoneme. An equal amount of protein (15 µg) was loaded on each lane in A and B. Numbers on the left of the gel show the positions of molecular mass markers (kDa). (C) Relative intensity of the 25 kDa band. The band intensities in the CC-treated sperm axoneme (white bars) and in the NH₄Cl-treated sperm axoneme (black bars) were compared. Band intensity was quantitated by densitometry and the maximum intensity of the band was taken as 1. Relative intensities of the 32 kDa band (D) and the 45 kDa band (E) are also shown.