Fig. 8. Analysis of F-actin level in enterocytes using laser-scanning cytometry. Representative experiment of the effect of (A) 5 nmol l^–1 MTX, (B) 75 nmol l^–1 palytoxin or (C) 75 nmol l^–1 ostreocin-D incubated for 4 h with intestinal cells in a Ca²⁺-free medium. MTX (A), palytoxin (B) and ostreocin-D (C) evoked a diminution in fluorescence intensity of treated cells, but this was less marked than when Ca²⁺ was present (see Fig. 9).