Fig. 5. RT-PCR to amplify chicken renal OAT1-like, OAT3-like, MRP2-like and MRP4-like cDNA. mRNA isolated from chick PTCs was reverse transcribed, and the resulting cDNA amplified using primers to chicken OAT1, OAT3, MRP2 and MRP4 (see Table 1). The RT-PCR products were run on a 2% agarose gel and stained with Gel-Star. The cDNA template was either present (+) or absent (–). No product was obtained when the cDNA template was omitted. Molecular size (base pairs) is indicated on the left.