Fig. 2. Indexes of total fatty acid unsaturation of membrane fractions. (A) Percentage of unsaturated fatty acids and unsaturation index of raft (triangles) and RDPM (squares) from 5°C-acclimated (open symbols) and 20°C-acclimated (filled symbols) fish. The dotted lines are isopleths representing the relationship between percentage unsaturation and unsaturation index if each unsaturated fatty acid had three (N=3) or four (N=4) double bonds. (B) Percentage of monounsaturated fatty acids (MUFA) and (C) percentage of polyunsaturated fatty acids (PUFA) in raft and RDPM from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) fish. Bars with a common lower case letter do not differ significantly. All values are means ± S.E.M., N=4. Raft, raft-enriched plasma membrane; RDPM, raft-depleted plasma membrane.