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Fig. 1. Raft-enriched plasma membrane (raft) and raft-depleted plasma membrane (RDPM) phospholipid class composition. Total lipids extracted from isolated membranes were separated by TLC and the major phospholipid classes were quantified by phosphate assay. (A) RDPM from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) trout. (B) Raft from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) trout. (C) RDPM (open bars) and raft (filled bars) from 5°C-acclimated trout. (D) RDPM (open bars) and raft (filled bars) from 20°C-acclimated trout. PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; SM, sphingomyelin. Values are means + S.E.M. (N=4).